Primer

Part:BBa_K5232003

Designed by: Dr. Ken Komiya (Extra Adviser), Fumiya Kashiwai   Group: iGEM24_UTokyo   (2024-09-27)

Usage and Biology

The 3' end strand has the complementary sequence to primer2, and the 5' end strand is the template strand for primer3 ^[1].

mechanism
Figure 1. Mechanism of TWJ > 3step-SDA
Result_123
Figure 2. The Result of TWJ > 3step-SDA

When TWJ-SDA was connected to 3step-SDA, an increase in amplification speed was observed around the 40-minute mark.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Komiya, K., Noda, C. & Yamamura, M. (2024). Characterization of Cascaded DNA Generation Reaction for Amplifying DNA Signal.New Gener. Comput. 42, 237-252. https://doi.org/10.1007/s00354-024-00249-2


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Categories
Parameters
None